Received 10.03.2023
DOI: 10.35556/idr-2023-2(103)22-27
Investigation of the effect of natural astaxanthin on cytotoxicity of mesenchymal stromal cells

Samoylova M.V.1, ORCID ID: 0000-0001-6771-919X, Kosyreva T.F.1, ORCID ID: 0000-0003-4333-5735, Samusenkov V.O.2, ORCID ID: 0000-0001-6266-0555, Grishaeva K.A.2, ORCID ID: 0000-0002-6932-9088, Voeykova O.V.1, ORCID ID: 0000-0003-2935-8965, Dragunova S.G.1, ORCID ID: 0000-0003-2497-2253
1 Peoples’ Friendship University of Russia, Department of Stomatology children’s age and orthodontics
Russia, 117198, Moscow, Miklukho-Maklaya St., 10/2
2 I.M. Sechenov’s First Moscow State Medical University (Sechenov’s University),
Russia, 119991, Moscow, Trubetskaya St., 8, Bld. 2.

E-mail address:

The search for new promising dental products that have anti-inflammatory, wound-healing, antibacterial and immunomodulatory effects and at the same time exhibit minimal side effects is quite an urgent task of modern scientific and practical medicine. As part of this work, we have produced four samples of an antioxidant dental gel containing natural astaxanthin. Astaxanthin, which is part of the dental gel, by its chemical structure belongs to the class of carotenoids and is a natural antioxidant present in various amounts in living organisms.
The first gel sample we made consisted of natural astaxanthin. An additional substance of the second sample of the preventive gel was interferon [12]. Interferon α has the ability to stimulate the phagocytic activity of macrophages, as well as the cytotoxic activity of T cells and NK cells. It has an indirect antiviral effect, increasing the body’s resistance to viral infections and modulating the immune system’s response aimed at neutralizing viruses or destroying infected cells. The third sample of dental gel contained polyprenol. Polyprenols are precursors of the most important transport lipid of all living organisms, dolichol. Since 2012, polyprenols have been included in the list of the most important essential components of nutrition for normal life support. Polyprenols are included in the list of vital components for the human body, as they are the building material for damaged cells. They help to stabilize cell membranes and restore cells of the brain, heart, liver and other organs, stimulate energy exchange and regenerative potential of body tissues. Polyprenols interact perfectly with antioxidants, increasing the efficiency of their work. The fourth sample included scallop extract as an enhancer of natural astaxanthin.
Objective: to evaluate the effect of antioxidant gel on the survival and proliferation of mesenchymal stromal cells (MSCs) of the human umbilical cord.
Materials and methods. Antioxidant gel with astaxanthin, astaxanthin with interferon, astaxanthin with polyprenol and astaxanthin with scallop extract were examined for the viability of MSCs in the MTT test and in the PI test.
Results. The antioxidant gel with interferon and polyprenol has a proliferative effect on MSCs compared to control samples, which confirms its potential of side effects, antitoxic and wound healing effect. When studying the induction of apoptosis, we found that the percentage of apoptotic cells according to the PI test did not differ from the cells of the control group, which may indicate the absence of apoptosis-inducing effect of drugs on mesenchymal stromal cells.
Conclusion. Studying the effects of the developed preventive antioxidant gel with astaxanthin, astaxanthin and interferon, astaxanthin and polyprenol, astaxanthin and scallop extract on the stromal cells of the human umbilical cord, no toxic and side effects were recorded.

Keywords: antioxidant gel, astaxanthin, interferon, polyprenol, scallop.

For citation: Samoylova M.V., Kosyreva T.F., Samusenkov V.O., Grishaeva K.A., Voeykova O.V., Dragunova S.G. Investigation of the effect of natural astaxanthin on cytotoxicity of mesenchymal stromal cells. Stomatology for All / Int. Dental Review. 2023, no.2(103): 22-27 (In Russian). doi: 10.35556/idr-2023-2(103)22-27
1. Sharma S., Venkatesan V., Prakhya B.M., Bhonde R. Human Mesenchymal Stem Cells as a Novel Platform for Simultaneous Evaluation of Cytotoxicity and Genotoxicity of Pharmaceuticals. Mutagenesis. 2015, 30: 391—399.
2. Pechinsky S.V., Kuregyan A.G., Oganesyan E.T., Stepanova E.F. Synthesis of lutein and astaxanthin es-ters and prediction of their activity. Journal of General Chemistry. 2019, 89(5): 721—725 (In Russian).
3. Capelli B., Talbott S., Ding L. Astaxanthin sources: Suitability for human health and nutrition. Functional Foods in Health and Disease. 2019, Jul 1; 9(6): 430—445.
4. Dong L.Y., Jin J., Lu G., Kang X.L. Astaxanthin at-tenuates the apoptosis of retinal ganglion cells in db/db mice by inhibition of oxidative stress. Marine Drugs. 2013, Mar, 11(3): 960—974.
5. Zhang Y., Yu J., Dong X.D., Ji H.Y. Research on characteristics, antioxidant and antitumor activities of dihydroquercetin and its complexes. Molecules. 2018, Jan, 23(1): 20. doi: 10.3390/molecules23010020
6. Hama S., Takahashi K., Inai Y., Shiota K., Sakamoto R., Yamada A, Tsuchiya H, Kanamura K, Yamashita E, Kogure K. Protective effects of topical application of a poorly soluble antioxidant astaxanthin liposomal formulation on ultraviolet-induced skin damage. Journal of pharmaceutical sciences. 2012, Aug 1; 101(8): 2909—2916. doi: 10.1002/jps.23216
7. Wu H., Niu H., Shao A., Wu C., Dixon B.J., Zhang J., Yang S., Wang Y. Astaxanthin as a potential neuro-protective agent for neurological diseases. Marine drugs. 2015, Sep, 13(9): 5750—5766. doi:
8. Ishiki M., Nishida Y., Ishibashi H., Wada T., Fujisa-ka S., Takikawa A., Urakaze M., Sasaoka T., Usui I., To-be K. Impact of divergent effects of astaxanthin on insu-lin signaling in L6 cells. Endocrinology. 2013, Aug 1, 154(8): 2600—2612.
9. Yoon K.D., Lee J.Y., Kim T.Y., Kang H., Ha K.S., Ham T.H., Ryu S.N., Kang M.Y., Kim Y.H., Kwon Y.I. In vitro and in vivo anti-hyperglycemic activities of taxifolin and its derivatives isolated from Pigmented Rice (Oryzae sativa L. cv. Superhongmi). J. Agric. Food Chem. 2019, Dec, 27, 68(3): 742—750. doi: 10.1021/acs.jafc.9b04962
10. Gamalei S.G., Bateneva A.V., Shishkina L.N., Skarnovich M.O., Medikova L.D., Bogryantseva M.P., Ivanova O.S., Levagina G.M., Danilenko E.D. Interferon-inducing and antiviral activity of a drug based on an in-terferon-gamma analog and an interferon inducer. Medi-cal immunology. 2017, 19(S): 378 (In Russian).
11. Roschina L.L. Pharmacy assortment of drugs of the interferon group and interferon inducers for the pre-vention and treatment of infectious diseases. Innovation. The science. Education. 2021, 29: 395—404 (In Russian).
12. Shvydkaya M.G., Zatevalov A.M., Mitrokhin S.D., Dzhandarova D.T., Mironov A.Yu. Combined effect of the peptide imunofan and moxifloxacin in vitro on the toxigenic strain of clostridium difficile. Clinical laboratory diagnostics. 2020, 65(8): 516—520 (In Russian). doi: 10.18821/0869-2084-2020-65-8-516-520