Received 07.01.2022
DOI: 10.35556/idr-2023-1(102)62-67
Optimization of integration of jaw bone tissue by cellular structures made of titanium impregnated with ascorbic acid
Vasilyuk V.P., Chetvertnykh V.A.
Federal State Budgetary Educational Institution of Higher Education “Academician Ye. A. Vagner Perm State Medical University” of the Ministry of Healthcare of the Russian Federation
Russia, 614990, Perm, Petropavlovskaya St., 26

Е-mail address: vasilyuk.vladimir53@mail.ru

Summary
The article presents a study on improving the efficiency of osseointegration processes in the plastic of jaw defects by using ascorbic acid in an experiment.
Structured implants with cells of 250 microns, obtained by growing on the Realizer SLM 50 installation, were implanted in guinea pigs in the lower jaw area (observation period of 1 month). For this purpose, 2 groups were formed: the main one — 5 animals and the comparison group — 3 animals. Ascorbic acid was a stimulant for the growth of connective and bone tissue. A method of introducing it into the implant cells has been developed. Studies were carried out on Altami MET-5D, a universal metallographic microscope used to work in reflected light at a magnification of 10 times.
As a result of the study, the IV degree of fixation prevailed in the main group (33.3±0.2%), the IV degree of fixation was also revealed in the comparison group (25±0.2%). Using an inductor (Vit. C) coarse-fibrous bone tissue was formed in implants with cells measuring 850 microns (term of 1 month), and in the absence of a modulator, connective tissue and cartilaginous tissue, penetrated by a network of small vessels, prevailed at the same time.
It is concluded that implants with a face-centered cubic crystal lattice structure have sufficiently high integration properties. At the same time, the use of vitamin C as a stimulant significantly increases the growth and integration of both connective and various types of bone tissue.

Keywords: osteointegration, cubic lattices, laser sintering, three-dimensional system, reflected light, coating bone tissue.

For citation: Vasilyuk V.P., Chetvertnykh V.A. Optimization of integration of jaw bone tissue by cellular structures made of titanium impregnated with ascorbic acid. Stomatology for All / Int. Dental Review. 2023, no.1(102): 62-67 (In Russian). doi: 10.35556/idr-2023-1(102)62-67

References
1. Believskaya R.R., Mingazeva A.S. Effect of ossein-hydroxyapatite complex on bone metabolism during dental implantation. Problems of dentistry. 2012, 5: 38—42 (In Russian).
2. Galonsky V.G., Radkevich A.A. Problems of replacement of mandibular defects in orthopedic dentistry. Siberian Medical Review. 2009, no.3(57): 18—23 (In Russian).
3. Mazzoli A. Selective laser sintering in biomedical engineering. Medical & Biological Engineering & Computing. 2013, 51(3): 245—256. doi: 10.1007/s11517-012-1001-x
4. Iryanov Yu.M., Iryanova T.Yu. Replacement of a bone defect in the conditions of transosseous osteosynthesis and the use of a titanium nickelide implant. Morphology. 2012, 142(5): 83—86 (In Russian).
5. Kuznetsova D.S., Timashev S.P., Bagratashvili V.N., Zagainova E.V. Bone implants based on scaffolds and cell systems in tissue engineering (Review). Modern technologies in the field of medicine. 2014, 6(4): 201—212 (In Russian).
6. Nazarov A.P., Okunkova A.A. Typical samples of products obtained by selective laser sintering. Bulletin of SSTU. 2012, no.3(67): 76—83 (In Russian).
7. Tverskoy M.M., Petrova L.N., Aladin S.A., Sulackaya E.Yu., Zharinova A.S. Computer technologies for manufacturing implants using the method of layer-by-layer laser sintering. Bulletin of the South Ural State University. 2012, 23(16): 64—69 (In Russian).
8. Rogozhnikov A.G., Gileva O.S., Hanov A.M., Shulyatnikova O.A., Rogozhnikov G.I., Pyankova E.S. The use of digital technologies for the manufacture of diaxidcirconium dentures, taking into account the individual parameters of the patient’s dental system. Rossijskij stomatologicheskij zhurnal. 2015, 19(1): 46—51 (In Russian).
9. Mullen L., Stamp R.C., Fox P., Jones E., Ngo C., Sutcliffe C.J. Selective laser melting: a unit cell approach for the manufacture of porous, titanium, bone in-growth constructs, suitable for orthopedic applications. II. randomized structures. Journal of Biomedical Materials Research — Part B Applied Biomaterials. 2010, 92(1): 178—188. doi: 10.1002/jbm.b.31504
10. Vasilyuk V.P., Shtraube G.I., Samusev I.V. Implant for replacement of mandibular defects. Patent for invention RF no.2469682. Bulletin no.35 issued at 20.12.2012 (In Russian).
11. Yangli Xu, Dongyun Zhang, Yan Zhou, Weidong Wang, Xuanyang Cao. Study on Topology Optimization Design, Manufacturability, and Performance Evaluation of Ti-6Al-4V Porous Structures Fabricated by Selective Laser Melting (SLM). Materials. 2017, 10(9): 1048. doi: 10.3390/ma10091048
12. Syam W.P., Mannan M.A., Al-Ahmari A.M. Rapid prototyping and rapid manufacturing in medicine and dentistry. Virtual and Physical Prototyping. 2011, 6(2): 79—109. doi: 10.1080/17452759.2011.590388
13. Vasilyuk V.P., Shtraube G.I., Chetvertnyh V.A. Optimization of surgical treatment of partial and complete jaw defects using cellular structures made of titanium in the experiment. Sovremennye problemy nauki i obrazovaniya. 2015, 19(1): 46—51 (In Russian).
14. Vasilyuk V.P., Shtraube G.I., Chetvertnyh V.A., Fajzrahmanov R.A., Dolgova E.V. A method for visualizing an individualized implant model for the replacement of bone defects in the jaws. Patent for invention RF no.2720167. Bulletin no.12 issued at 24.04.2020 (In Russian).
15. Vasilyuk V.P., Shtraube G.I., Chetvertnyh V.A., Kilina P.N., Kocherzhuk S.A. An implant for the replacement of jaw defects after the removal of near-root cysts. Patent for invention RF no.2612123. Bulletin no.7 issued at 02.03.2017 (In Russian).