Received 28/02/2024

DOI: 10.35556/idr-2024-3(108)68-72
The effect of local hardware hypothermia on microcirculation in vivo
Chromenkova K.V.1, ORCID ID: 0000-0001-8230-0258,
Taranova N.Y.2, ORCID ID: 0009-0006-7313-3543, SPIN-code: 9091-2134,
Belova N.M.1, SPIN-code: 1539-0670,
Saperova N.R.1, SPIN-code: 8533-4020,
Gusarov A.M.3, ORCID ID: 0000-0002-6583-4685; Author ID: 1089978,
Ivanova I.V.3, ORCID ID: 0000-0002-8244-4136, SPIN-code: 9271-7500, Author ID: 1185201

1Federal State Budgetary Educational Institution of Further Professional Education «Russian Medical Academy of Continuous Professional Education» of the Ministry of Health of the Russian Federation
125993, Russia, Moscow, Barrikadnaya St., 2/1, Bld.1
2Federal State Budgetary Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation
350063, Russia, Krasnodarskiy kray, Krasnodar, Mitrofana Sedina St., 4
3I.M. Sechenov First Moscow State Medical University (Sechenov University), E.V. Borovsky Institute of Dentistry, Department of Maxillofacial Surgery named after Academician N.N. Bazhanov
119048, Russia, Moscow, Trubetskaya St., 8, Bld. 2

E-mail address: khromenkovakv@mail.ru

Summary
Purpose. Demonstrate microcirculatory changes and justify the effectiveness of controlled hardware hypothermia in an experiment
Material and methods. To study microcirculatory changes during local instrumental hypothermia, everted cheek pouches of 20 hamsters were used. Local hardware hypothermia (LHH) was carried out using the ViThermo device in a mode with a target temperature of 18°C for 60 minutes. Optical coherence tomography (OCT) was performed for intravital monitoring of microcirculatory changes.
Results. At an interval of up to 5 min. There was an increase in vascular density to a value of 5.31% and a decrease to 4.76%. At the same time, the peak vascular system density (VSD) was reached at 3 minutes of LHH (5.31%). After 5 minutes of LHH application, and subsequently until 60 minutes, VSD decreased in steps from 0.1% to 0.02% per 1 minute per step. The lowest VSD was achieved at 57-60 minutes of VSD. The temperature of the outer surface of the golden hamster’s cheek pouch decreased slowly and gradually in increments of 1°C to 0.1°C in 1-10 minutes. After 60 minutes of LHH, the temperature of the golden hamster’s cheek pouch began to increase: at step 61 minutes, 19.80°C and 62 minutes, 21.40°C. VSD at 61-62 minutes stabilized to values of 3.62% and 4.43%, respectively.
Conclusions. The effect of LHH on the microvascular bed of the hamster buccal mucosa revealed a number of characteristic physiological changes. With a gradual decrease in tissue temperature, a slow decrease and then an increase in vascular VSD was detected on OKT scans. This substantiates the hypothesis that the use of “soft” tissue cooling in the form of PAH is more rational in relation to the microcirculation.

Keywords: local hardware hypothermia, optical coherence tomography, microcirculation.

For citation: Chromenkova K.V., Taranova N.Y., Belova N.M., Saperova N.R., Gusarov A.M., Ivanova I.V. The effect of local hardware hypothermia on microcirculation in vivo. Stomatology for All / Int. Dental Review. 2024; no.3(108): 68-72 (in Russian). doi: 10.35556/idr-2024-3(108)68-72

References
1. Possoff A. External thermal applications in postextraction therapy. Journal of the American Dental Association. 1955; 50, no. 2: 147–56. doi: 10.14219/jada.archive.1955.0040
2. Ebrall P.S., Bales G.L., Frost B.R. An improved clinical protocol for ankle cryotherapy. J Manual Medicine. 1992; 6: 161–165.
3. Moro A., Gasparini G., Marianetti T.M., Boniello R., Cervelli D., Di Nardo F., et al. Hilotherm efficacy in controlling postoperative facial edema in patients treated for maxillomandibular malformations. The Journal of craniofacial surgery. 2011; 22(6): 2114–2117. doi: 10.1097/SCS.0b013e31822e5e06
4. Friscia M., et al. Efficacy of Hilotherapy face mask in improving the trend of edema after orthognathic surgery: a 3D analysis of the face using a facial scan app for iPhone. Oral and maxillofacial surgery. 2021; 26: 485–490. doi: 10.1007/s10006-021-01015-0
5. Glass G.E., Waterhouse N., Shakib K. Hilotherapy for the management of perioperative pain and swelling in facial surgery: A systematic review and meta-analysis. The British Journal of Oral & Maxillofacial Surgery. 2016; 54(8): 851-856.
6. Greenstein G. Therapeutic efficacy of cold therapy after intraoral surgical procedures: a literature review. J Periodontol. 2007; 78: 790Y800.
7. Sortino F., Messina G., Pulvirenti G. Rilevazioni di temperatura mucosa e cutanea nel decorso postoperatorio di terzi molari inclusi [Evaluation of postoperative mucosa and skin temperature after surgery for impacted third molar]. Minerva stomatologica. 2003; 52(7-8): 393–399 (in Italian).
8. do Nascimento-Junior E.M., dos Santos G.M.S., Tavares Mendes M.L., Cenci M., Correa M.B., Pereira-Cenci T., et al. Cryotherapy in reducing pain, trismus, and facial swelling after third-molar surgery: Systematic review and meta-analysis of randomized clinical trials. Journal of the American Dental Association. 2019; 150(4): 269–277.e1. doi: 10.1016/j.adaj.2018.11.008
9. Penarrocha M., Sanchis J.M., Saez U., Gay C., Bagan J.V. Oral hygiene and postoperative pain after mandibular third molar surgery. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001; 92(3): 260–264.
10. Song J., Kim H., Park E., et al. Pre-emptive ice cube cryotherapy for reducing pain from local anaesthetic injections for simple lacerations: a randomised controlled trial. Emerg Med J. 2018; 35(2): 103–107.
11. Renton T. An update on pain. Br Dent J. 2008; 204(6): 335–338.
12. Zheng X., Luo S., Huang C., Wang Z., Lin X. Comparison of kinesiology tape and cryotherapy on postoperative reaction following third molar extraction: a randomized clinical trial. Quintessence international. 2022; 53(9): 772–777. doi: 10.3290/j.qi.b3314957