Received 27.09.2025

DOI: 10.35556/idr-2025-4(113)42-49

Assessment of the influence of calcium phosphate-containing and fluoride-containing hygienic products on the level of mineralization of enamel of teeth with fluorosis using structural and elemental analysis in vitro
Klevtsova S.O.1, ORCID: 0000-0003-1778-3972
Kiselnikova L.P.1, ORCID: 000-0003-2095-9473
Vasiliev A.V.2, ORCID: 0000-0002-7169-2724
Mironov A.V.3, ORCID: 0000-0002-8173-0253
Daskal E.A.4, ORCID: 0009-0003-3258-8718
1 Federal State Budgetary Educational Institution of Higher Education “Russian University of Medicine” of the Ministry of Health of the Russian Federation
127006, Russia, Moscow, Dolgorukovskaya St., 4
2 National Medical Research Center for Dentistry and Maxillofacial Surgery of the Ministry of Health of Russia
119021, Russia, Moscow, Timura Frunze St., 16
3 National Research Centre “Kurchatov Institute”
123182, Russia, Moscow, Akademika Kurchatova Sq.,1
4 Krasnogorsk Dental Clinic named after L.F. Smurova
143401, Russia, Krasnogorsk, Moskovskaya obl., Il’inskoye Shosse, 4

E-mail address: lpkiselnikova@mail.ru

Summary
Due to the increasing prevalence among children of a dental developmental defect such as fluorosis, this issue attracts significant attention from dental specialists. In some regions of Russia, the prevalence of fluorosis among children reaches 40–43 % due to elevated fluoride content in drinking water.
The aim of the study is to assess how calcium phosphate-containing and fluoride-containing hygienic products and preparations affect the enamel of teeth with fluorosis.
Material and methods. An experiment was conducted using structural and elemental analysis in vitro.
The study objects were premolars extracted for orthodontic reasons with undeveloped roots and mild fluorosis in children aged 12–14 years. The control group consisted of premolars without fluorosis, extracted for orthodontic reasons from children of the same age living in areas with optimal fluoride content in drinking water.
For the analysis of enamel morphology and the distribution of calcium, fluoride, and phosphorus within it, a scanning electron microscope and energy-dispersive X-ray analysis were used.
Results and discussion. Showed that the use of fluoride-containing agents leads to a significant increase in the fluoride content in the enamel. Calcium phosphate-containing preparations, in turn, increase the calcium level in the enamel of fluorosed teeth almost to the level’s characteristic of teeth without fluorosis.
Conclusion. The combined use of fluoride- and calcium phosphate-containing agents ensures a more uniform and physiological distribution of these elements in the superficial and deep layers of the enamel, which may be important in determining the level of caries resistance of tooth enamel.

Keywords: dental fluorosis, enamel mineralization, calcium phosphate-containing agents, fluoride-containing agents, remineralization, scanning electron microscopy, energy-dispersive X-ray analysis.

For citation: Klevtsova S.O., Kiselnikova L.P., Vasiliev A.V., Mironov A.V., Daskal E.A. Assessment of the influence of calcium phosphate-containing and fluoride-containing hygienic products on the level of mineralization of enamel of teeth with fluorosis using structural and elemental analysis in vitro. Stomatology for All / Int. Dental Review. 2025; no. 4 (113): 42–49 (in Russian). doi: 10.35556/idr-2025-4(113)42-49

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