Received 30.05.2025

DOI: 10.35556/idr-2025-3(112)48-53

The effectiveness of the prevention of dental caries by combinations of oral hygiene products with aminofluoride and nanohydroxyapatite
Samatova R.Z., ORCID: 0000-0002-9549-2911
Fakhrutdinova L.M., ORCID: 0000-0001-9506-563X
Timerbulatova G.A.
Saleev R.A., ORCID: 0000-0003-3604-7321
Shiryak T.Yu., ORCID: 0000-0003-4546-4784
Saleeva G.T., ORCID: 0000-0001-9751-0637
Federal State Budgetary Educational Institution of Higher Education “Kazan Medical University” of the Ministry of Health of the Russian Federation
420012, Russia, Kazan, Butlerova St., 49

E-mail address: tanya_shiryak@mail.ru

Summary
The comparative effectiveness of nanohydroxyapatite (nanoHAP) and fluoride oral hygiene products is debatable. Some researchers point to the advantage of nanoHAP; other researchers claim the advantage of fluorides, while others do not identify significant differences between them. Most nanoHAP- studies are in vivo and have been studied for a short time; there are not enough studies investigating the effectiveness of combinations of basic and additional oral hygiene products. These problems were the basis for our research.
The aim: to study the effectiveness of dental caries prevention with various combinations of nanoHAP and aminofluoride oral hygiene products.
Material and methods: 140 children (7–9 years old) were examined before and after preventive measures. Three groups were formed: group 1 used aminofluoride-toothpaste and nanoHAP-mouthwash (46 children); group 2 used nanoHAP-toothpaste and nanoHAP- mouthwash (46 children), group 3 used aminofluoride-toothpaste (48 children). We used the following indices to evaluate the effectiveness of caries prevention: DMFS-indices, intensity increase of caries (ΔDMF), caries reduction, the enamel acid resistance test and the saliva microcrystallization test.
Results and discussion: After a year of preventive measures, the intensity increase of caries in the 1st group was ΔDMF=0.3 (32 % caries reduction); in the second group the intensity increase of caries was ΔDMF=0.52; in the third group the intensity increase of caries was ΔDMF=0.45 (13 % caries reduction). The values of the enamel acid resistance test and the saliva microcrystallization test by the 9th month were significantly higher in the 1st group.
Conclusion. Fluorides must necessarily be included in the individual oral hygiene for the prevention of dental caries in children. A combination of oral hygiene products aminofluoride- toothpaste and nanoHAP-mouthwash provides a greater caries reduction and changes the enamel acid resistance test and the saliva microcrystallization test, more effectively than a combination of nanoHAP-toothpaste with and nanoHAP-mouthwash or aminofluoride-toothpaste without mouthwash.

Keywords: nanohydroxyapatite, aminofluoride, oral hygiene products, prevention of dental caries, toothpaste, mouthwash.

For citation: Samatova R.Z., Fakhrutdinova L.M., Timerbulatova G.A., Saleev R.A., Shiryak T.Yu., Saleeva G.T. The effectiveness of the prevention of dental caries by combinations of oral hygiene products with aminofluoride and nanohydroxyapatite. Stomatology for All / Int. Dental Review. 2025; no. 3 (112): 48–53 (in Russian). doi: 10.35556/idr-2025-3(112)48-53

References
1. Mitropanova M.N., Pavlovskaya O.A., Zneibat M.S., Sinitsina N.S. Influence of the buffering system on hard dental tissue remineralization. Pediatric dentistry and dental prophylaxis. 2018; no. 17 (2): 71–76 (in Russian). doi: 10.25636/PMP.3.2018.2.13
2. Abou Neel E.A., Aljabo A., Strange A., Ibrahim S., Coathup M., Young A.M. et al. Demineralization-remineralization dynamics in teeth and bone. Int J Nanomedicine. 2016; no. 11: 4743–4763. doi: 10.2147/IJN.S107624
3. Sejdini M., Meqa K., Berisha N., Çitaku E., Aliu N., Krasniqi S., Salihu S. The Effect of Ca and Mg Concentrations and Quantity and Their Correlation with Caries Intensity in School-Age Children. Int J Dent. 2018; doi: 10.1155/2018/2759040
4. Smetanin A.A., Ekimov E.V., Skripkina G.I. Ion-exchange processes in the tooth enamel and means of enamel remineralization (the literary review). Pediatric dentistry and dental prophylaxis. 2020; no. 20 (1):77–80 (in Russian). doi: 10.33925/1683-3031-2020-20-1-77-80
5. Homenko L.A., Sorochenko G.V., Ostapko E.I., Biden-ko N.V., Savychuk A.V., Golubeva I.N. Experimental substantiation of the management of minee-ramping processes of emali permanent teeth. Modern dentistry. 2020, no. 1 (78): 48–53 (in Russian).
6. Amaechi B.T., Alshareif D.O., Azees P.A.A., Shehata M.A., Lima P.P., Abdollahi A. et al. Anti-caries evaluation of a nanohydroxyapatite dental lotion for use after toothbrushing: An in situ study. J Dent. 2021; no. 115: 103863. doi: 10.1016/j.jdent.2021.103863
7. Manchery N., John J., Nagappan N., Subbiah G.K., Premnath P. Remineralization potential of dentifrice containing nanohydroxyapatite on artificial carious lesions of enamel: A comparative in vitro study. Dent Res J (Isfahan). 2019; no. 16 (5): 310–317.
8. Shaw L., Murray J.J., Burchell C.K., Best J.S. Calcium and phosphorus content of plaque and saliva in relation to dental caries. Caries Res. 1983; no. 17 (6): 543–548. doi: 10.1159/000260715
9. Abedi M., Ghasemi Y., Nemati M.M. Nanotechnology in toothpaste: Fundamentals, trends, and safety. Heliyon. 2024; no. 10 (3): p 21. doi: 10.1016/j.heliyon.2024.e24949
10. Kani T., Kani M., Isozaki A. The effect of apatite–containing dentifrices on artificial caries lesions. J of the Japanese Society of Dental Health. 1988, no. 38: 364–365.
11. Amaechi B.T., Phillips T.S., Evans V., Ugwokaegbe C.P., Luong M.N., Okoye L.O. et al. The Potential of Hydroxyapatite Toothpaste to Prevent Root Caries: A pH-Cycling Study. Clin Cosmet Investig Dent. 2021; no. 13: 315–324. doi: 10.2147/CCIDE.S319631
12. Limeback H., Enax J., Meyer F. Biomimetic hydroxyapatite and caries prevention: a systematic review and meta-analysis. Can J Dent Hyg. 2021; no. 55 (3): 148–159.
13. Enax J., Fabritius H-O., Fabritius-Vilpoux K., Amaechi B.T., Meyer F. Modes of action and clinical efficacy of particulate hydroxyapatite in preventive oral health care – state of the art. Fabritius-Vilpoux Open Dent. J. 2019, no. 13: 274–287.
14. Kensche A., Holder C., Basche S., Tahan N., Hannig C., Hannig M. Efficacy of a mouthrinse based on hydroxyapatite to reduce initial bacterial colonisation in situ. Arch Oral Biol. 2017; no. 80: 18–26. doi: 10.1016/j.archoralbio.2017.03.013
15. Dobrota C.T., Florea A.D., Racz C.P., Tomoaia G., Soritau O., Avram A. et al. Dynamics of Dental Enamel Surface Remineralization under the Action of Toothpastes with Substituted Hydroxyapatite and Birch Extract. Materials (Basel). 2024; no. 17 (9): 2038. doi: 10.3390/ma17092038
16. Toothpaste based on hydroxyapatite “Parodontol”. Stomatology for All / Int. Dental Review. 2025; no. 1 (2): 42–43 (in Russian). doi: 10.35556/idr-2025-1(2)42-43
17. Juntavee A., Juntavee N., Hirunmoon P. Remineralization Potential of Nanohydroxyapatite Toothpaste Compared with Tricalcium Phosphate and Fluoride Toothpaste on Artificial Carious Lesions. Int J Dent. 2021; no. 2021: 5588832. doi: 10.1155/2021/5588832
18. de Carvalho F.G., Vieira B.R., Santos R.L., Carlo H.L., Lopes P.Q., de Lima B.A. In vitro effects of nano-hydroxyapatite paste on initial enamel carious lesions. Pediatr Dent. 2014; no. 36 (3): 85–89.
19. Ebadifar A., Nomani M., Fatemi S.A. Effect of nano-hydroxyapatite toothpaste on microhardness ofartificial carious lesions created on extracted teeth. J Dent Res Dent Clin Dent Prospects. 2017; no. 11 (1): 14–17. doi: 10.15171/joddd.2017.003
20. Geeta R.D., Vallabhaneni S., Fatima K. Comparative evaluation of remineralization potential of nanohydroxyapatite crystals, bioactive glass, casein phosphopeptide-amorphous calcium phosphate, and fluoride on initial enamel lesion (scanning electron microscope analysis) – An in vitro study. J Conserv Dent. 2020; no. 23 (3): 275–279. doi: 10.4103/JCD.JCD_62_20
21. Grewal N., Sharma N., Kaur N. Surface remineralization potential of nano-hydroxyapatite, sodium monofluorophosphate, and amine fluoride containing dentifrices on primary and permanent enamel surfaces: An in vitro study. J Indian Soc Pedod Prev Dent. 2018; no. 36 (2): 158–166. doi: 10.4103/JISPPD.JISPPD_142_17
22. Paszynska E., Pawinska M., Gawriolek M., Kaminska I., Otulakowska-Skrzynska J., Marczuk-Kolada G. et al. Impact of a toothpaste with microcrystalline hydroxyapatite on the occurrence of early childhood caries: a 1-year randomized clinical trial. Sci Rep. 2021; no. 11 (1): 2650. doi: 10.1038/s41598-021-81112-y
23. Thimmaiah C., Shetty P., Shetty S.B., Natarajan S., Thomas N.A. Comparative analysis of the remineralization potential of CPP-ACP with Fluoride, Tri-Calcium Phosphate and Nano Hydroxyapatite using SEM/EDX – An in vitro study. J Clin Exp Dent. 2019; no. 11 (12): 1120–1126. doi: 10.4317/jced.55941
24. Brambilla E., Ionescu A., Cazzaniga G., Edefonti V., Gagliani M. The influence of antibacterial toothpastes on in vitro Streptococcus mutans biofilm formation: a continuous culture study. Am J Dent. 2014; no. 27 (3): 160–1666.
25. Chandru T.P., Yahiya M.B., Peedikayil F.C., Dhanesh N., Srikant N., Kottayi S. Comparative evaluation of three different toothpastes on remineralization potential of initial enamel lesions: A scanning electron microscopic study. Indian J Dent Res. 2020; no. 31 (2): 217–223. doi: 10.4103/ijdr.IJDR_745_18
26. Esteves-Oliveira M., Santos N.M., Meyer-Lueckel H., Wierichs R.J., Rodrigues J.A. Caries-preventive effect of anti-erosive and nano-hydroxyapatite-containing toothpastes in vitro. Clin Oral Investig. 2017; no. 21 (1): 291–300. doi: 10.1007/s00784-016-1789-0
27. Amaechi B.T., AbdulAzees P.A., Alshareif D.O., Shehata M.A., de Carvalho Sampaio Lima P.P., Abdollahi A. et al. Comparative efficacy of a hydroxyapatite and a fluoride toothpaste for prevention and remineralization of dental caries in children. BDJ Open. 2019, no. 5: 18. doi: 10.1038/s41405-019-0026-8
28. Nishimuta M., Kodama N., Yoshitake Y., Shimada M., Serizawa N. Dietary Salt (Sodium Chloride) Requirement and Adverse Effects of Salt Restriction in Humans. J Nutr Sci Vitaminol (Tokyo). 2018; no. 64 (2): 83–89. doi: 10.3177/jnsv.64.83. PMID: 29710036
29. Pajor K., Pajchel L., Kolmas J. Hydroxyapatite and Fluorapatite in Conservative Dentistry and Oral Implantology-A Review. Materials (Basel). 2019; no. 12 (17): 2683. doi: 10.3390/ma12172683
30. Yang J., Yuan P., Liu C., Liu P., Ning H., Xu P. Effect of nanohydroxyapatite on surface mineralization in acid-etched dentinal tubules and adsorption of lead ions. Nan Fang Yi Ke Da Xue Xue Bao. 2020; no. 40 (9): 1307–1312. doi: 10.12122/j.issn.1673-4254.2020.09.13
31. Enax J., Epple M. Synthetic Hydroxyapatite as a Biomimetic Oral Care Agent. Oral Health Prev Dent. 2018; no. 16 (1): 7–19. doi: 10.3290/j.ohpd.a39690