Parametric assessment of lactoferrin activity in experiment and clinical application
Tsarev V.N.1, https://orcid.org/0000-0002-3311-0367, Podporin M.S.1, https://orcid.org/0000-0001-6785-0016, Sadchikova E.R.2, http://orcid.org/0000-0003-2039-7108, Trefilova Yu.A.1, https://orcid.org/0000-0001-9571-0520, Arutyunyan A.A.1, Ezhova A.V.1, Goldman I.L.2, http://orcid.org/0000-0003-1534-3367
1 Federal State Budgetary Educational Institution of Higher Education “A.I. Yevdokimov Moscow State University of Medicine and Dentistry” of the Ministry of Healthcare of the Russian Federation
Russia, 127473, Moscow, Delegatskaya St., 20, bld. 1
2 Institute of Gene Biology Russian Academy of Sciences Ministry of Science and Higher Education of the Russian Federation.
Russia, 119334, Moscow, Vavilova St., 34/5
E-mail address: email@example.com
The possibilities of using lactoferrin preparations for the destruction of microbial biofilms as the leading factors in the development of oral diseases are considered.
Materials and methods. As part of the experiment, a microbiological diagnostic technique was used using a system for the automatic cultivation of microbial populations. A pre-prepared bacterial suspension was inoculated into the nutrient broth and the studied lactoferrin samples were added, followed by cultivation and analysis of the possible antibacterial effects of transferrin protein. The infrastructure of the Unique scientific installation “Transgenbank” was used in the work. The assessment of the growth control of the corresponding type of bacteria was reflected in the change in the optical density parameters, on the basis of which the curve was constructed.
Results. According to the results of an experimental study of the growth curves of bacterial populations, statistically significant differences in the number of viable cells in different phases of the growth curves were noted. The analysis of growth dynamics revealed differences in the onset of maximum reproduction and its inhibition under the influence of various aggravating factors during cultivation. The bacteriostatic effect of lactoferrin is realized by binding iron ions, depriving bacteria of this trace element, causing inhibition of their development.
Conclusion. With local application of lactoferrin-containing drug in the complex periodontal treatment of patients, normalization of the clinical picture of periodontitis was established, a statistically significant decrease in the indices of OHI-S, PHP, PMA, PBI and SBI indices in the dynamics of clinical observation, which remained optimal for 1 year. The used bioreactor allows to obtain reproducible results, is available for wide use and can be recommended for obtaining objective, comparable, reliable information about the antimicrobial properties of various samples of the bactericidal protein lactoferrin produced by the domestic pharmaceutical industry.
Keywords: lactoferrin, bioreactor, cultivation, growth curve, periodontal treatment, pharmaceutical industry.
For citation: Tsarev V.N., Podporin M.S., Sadchikova E.R., Trefilova Yu.A., Arutyunyan A.A., Ezhova A.V., Goldman I.L. Parametric assessment of lactoferrin activity in experiment and clinical application. Stomatology for All / Int. Dental Review. 2021, no.4(97): 59-65 (In Russian). doi: 10.35556/idr-2021-4(97)59-65
1. Balmasova I.P., Tsarev V.N., Yanushevich O.O., Mayev I.V., Mkrtumyan A.M., Arutyunov S.D. Microecology of periodontal disease. The relationship of local and systemic effects. Moscow: Practical Medicine, 2021, 264 p. (In Russian).
2. Ippolitov E.V., Didenko L.V., Tsarev V.N. Morphology features of periodontal biofilm in inflammatory gum diseases (chronic catarrhal gingivitis, chronic periodontitis, candida-associated periodontitis) according to electron microscopy. Clinical laboratory diagnostics. 2015, 60, 12: 59—64 (In Russian).
3. Pinegina O.N., Raush E.R., Vasilyeva N.V. Determination of sensitivity to Candida spp antimycotics. as part of biofilms. Problems of medical mycology. 2014, no.4(16): 46—48 (In Russian).
4. Ushakov R.V., Tsarev V.N., Ashueva A.Zh., Ushakov A.R., Tsareva T.V. Antimicrobial prevention of local inflammatory complications in surgical dentistry. Stomatology for All / Int. Dental Review. 2021, no.3(96): 4—11 (In Russian). doi: 10.35556/idr-2021-3(96)04-11
5. Gendreau L., Loewy Z.G. Epidemiology and etiology of denture stomatitis. J Prosthodont. 2011, 20: 251—260. https://doi.org/10.1111/j.
6. Gleiznys A., Zdanaviciene E., Ziilinskas J. Candida albicans importance to denture wearers. A literature review. Stomatol Balt Dent Maxillofac J. 2015, 17: 54—66.
7. Bukharin O.V., Valyshev A.V., Valysheva I.V. The role of lactoferrin in anti-infective protection. The successes of modern biology. 2011, 2(131): 135—144 (In Russian).
8. Kuznetsov I.A., Potievskaya V.I., Kachanov I.V. Study of iron-containing proteins (lactoferrin, ferritin) in physiological conditions and in laboratory diagnostics of diseases. In: Gulyaev G.Yu. Innovative development: the potential of science and modern education. Penza: Science and Education, 2017: 156—165 (In Russian).
9. Akiyama Y., Oshima K., Kuhara T., Shin K. lactoferrin-receptor, intelectin 1, affects uptake, sub-cellular localization and release of immunochemically detectable lactoferrin by intestinal epithelial Caco-2 cells. Journal of Biochemistry. 2013, 154(5): 437—448.
10. Goldman I.L., Georgieva S.G., Gurskiy Y.G., Krasnov A.N., Deykin A.V., Popov A.N., Ermolkevich T.G., Budzevich A.I., Chernousov A.D., Sadchikova E.R. Production of human lactoferrin in animal milk. Biochemistry Cell Biology. 2012, 90(3): 513—519.
11. Tsarev V.N., Goldman I.L., Sadchikova E.R., Ippolitov E.V., Podporin M.S. Evaluation of the effect of recombinant human lactoferrin on the characteristics of growth curves of bacterial populations of pathogens. National priorities of Russia. 2016, no.4(22): 130—133 (In Russian).
12. Sujata S., Mau S., Sanket K., Punit K., Tej P. C-Lobe of Lactoferrin: The Whole Story of the Half-Molecule. Biochemistry research international. 2013: 1—8.
13. Cornish J., Naot D. Lactoferrin as an effector molecule in the skeleton. Biometals. 2010, 23(3): 425—430.