Received 15.02.2025
DOI: 10.35556/idr-2025-1(110)70-76
Use of Raman fluorescence diagnostics method to mineralization study of dental hard tissues in non-carious lesions and after removal of bracket systems
Altynova E.E.1, ORCID: 0000-0001-7544-2303
Aleksandrov M.T.1, ORCID: 0000-0003-2777-296X
Mamedova L.A.1, ORCID: 0000-0002-8768-7444
Bashtovoy А.А.2, ORCID: 0000-0003-3642-967X
Nesterova K.I.3, ORCID: 0000-0002-9793-7179
Pilipenko A.V.1, ORCID: 0009-0008- 5475-1543
1 Moscow Regional Research and Clinical Insitute (MONIKI)
129110, Moscow, Russia, Shchepkin st., 61/2
2 Central state medical academy of department of presidential affairs
121359, Russia, Moscow, Marshala Timoshenko st.,19, bld. 1A
3 Federal State Budgetary Educational Institution of Higher Education “Omsk State Medical University” of the Ministry of Health of the Russian Federation
644099, Russia, Omsk, Lenina st., 12
E-mail address: pulsar.88@icloud.com
Summary
Rehabilitation of patients undergoing orthodontic treatment with subsequent detection of enamel demineralization foci is one of the leading problems of modern orthodontics.
Material and methods. 90 patients were monitored, who were grouped into 6 groups: 1 – patients with intact teeth, 2 – with enamel demineralization after removal of braces, 3 – with wedge–shaped defects, 4 – with dental fluorosis, 5 – with pathological erasability, 6 – patients with hypoplasia enamel. Mineralization indicators in all groups reflected the level of mineralization both at the intact point (object of comparison) and in the zone of its violation (object of observation).
Results. In 90 patients with demineralization of hard dental tissues in the initial caries, enamel hypoplasia, wedge-shaped defects, fluorosis foci and increased abrasion, the digital method of Raman fluorescence diagnostics revealed a statistically significant decrease in mineralization by 10–300 % in relation to the intact areas of the tooth, with the following indicators: for intact teeth 60,5–62,1 relative units, for initial caries 60,5–53 relative units, for wedge-shaped defect 84,5–72 relative units, for fluorosis foci 111,6–113 relative units, for teeth with increased abrasion 199,1–72,6 relative units in the foci. The decrease in the indicators was in all cases except for teeth with fluorosis, where it was higher than the indicators of intact teeth by almost 2 times, and for teeth with increased abrasion – 3 times. Based on the obtained results, a clinical algorithm for assessing the mineralization of hard dental tissues using the Raman fluorescence diagnostics method was developed.
Conclusion. The developed digital medical technology is a promising direction for assessing the condition of hard dental tissues in normal and pathological conditions, and will also contribute to the effective rehabilitation process of patients with foci of demineralization of various etiologies.
Keywords: demineralization, bracket system, non-carious lesions, Raman fluorescence technologies.
For citation: Altynova E.E., Aleksandrov M.T., Mamedova L.A., Bashtovoy А.А., Nesterova K.I., Pilipenko A.V. Use of Raman fluorescence diagnostics method to mineralization study of dental hard tissues in non-carious lesions and after removal of bracket systems. Stomatology for All / Int. Dental Review. 2025; no. 1 (110): 70–76 (in Russian). doi: 10.35556/idr-2025-1(110)70-76
References
1. Martignon S., Bartlett D., Manton D.J., Martinez-Mier E.A., Splieth C., Avila V. Epidemiology of Erosive Tooth Wear, Dental Fluorosis and Molar Incisor Hypomineralization in the American Continent. Caries Res. 2021; no. 55 (1): 1–11. doi: 10.1159/000512483
2.Aleksandrov M.T., Podoynikova M.N., Eganyan D.G. Assessment of mineralization of hard tissues of the tooth by the method of Raman-fluorescent medical technologies under the influence of various physical and chemical factors in patients in norm and carious and non-carious lesions. Russian journal of dentistry. 2023; no. 27 (6): 521–531 (in Russian). doi: 10.17816/dent508778
3. Chesnokov V.A., Chesnokova M.G., Nesterova K.I., Musienko A.I. Evaluation of hygienic state of oral cavity in persons with excess body mass index at the stages of orthopedic rehabilitation with removable prostheses. Periodontology. 2019; no. 24 (3): 269–273 (in Russian). doi: 10.33925/1683-3759-2019-24-3-269-273
4.Iordanishvili A.K., Soldatova L.N., Soldatov S.V., Zuikova M.A., Soldatov V.S. Characteristics of periodontal tissues and oral hygiene in elderly people with comorbid pathology and ways to improve it. Periodontology. 2018; no. 24 (4): 4–8 (in Russian). doi:10.25636/PMP.1.2018.4.1
5. Polyakova R.V., Nikolaev N.A., Nedoseko V.B. Composition of dentoliths in patients with somatic pathology. Periodontology. 2018; no. 24 (4): 56–60 (in Russian). doi: 10.25636/PMP.1.2018.4.10
6. Montasser M.A., El-Wassefy N.A., Taha M. In vitro study of the potential protection of sound enamel against demineralization. Progress in Orthodontics. 2015; 16, no. 12: 1–6. doi: 10.1186/s40510-015-0080-2
7.Musienko A.I., Nesterova K.I., Musienko A.A. Prophylactic hygienic measures after reparative osteogenesis in patients with chronic generalized periodontitis. Periodontology. 2019; no. 24 (2): 179–183 (in Russian). doi:10.33925/1683-3759-2019-24-2-179-183
8. Fedorova K.V., Gavrilova O.A., Zatevalov A.M., Mironov A.Yu. Forecast of caries intensity dynamics when using different orthodontic appliances. Certificate of registration of pro-gram for computer RU 2023615120, 10.03.2023. Application № 2023613583 from 20.02.2023 (in Russian).
9. Barrera-Ortega C.C., Vazquez-Olmos A.R., Sato-Berru R.Y., Araiza-Tellez M.A. Study of demineralized dental enamel treated with different fluorinated compounds by Raman spectroscopy. Journal of Biomedical Physics and Engineering. 2020; no. 10 (5): 635–644. doi: 10.31661/jbpe. v0i0.2003-1089
10. Orekhova L.Y., Loboda E.S., Neizberg D.M., Boeva P.A., Berezkina I.V. Comparative evaluation of changes in the ultrastructure of the enamel surface of teeth and dental implants when using air-abrasive techniques of surface decontamination with different types of abrasives in the process of professional hygiene. Periodontology. 2019; no. 24 (2): 133–139 (in Russian). doi: 10.33925/1683-3759-2019-24-2-133-139
11.Magsumova O.A., Polkanova V.A., Timchenko E.V., Volova L.T. Raman spectroscopy and its application in stomatology. Stomatology. 2021; no. 100 (4): 137–142 (in Russian). doi: 10.17116/stomat2021100041137
12. Aleksandrov M.T., Kukushkin V.I., Polyakova M.A., Novozhilova N.E., Babina K.S., Arakelyan M.G. et al. Raman-fluorescence characteristics of hard dental tissues and their clinical significance. Russian journal of dentistry. 2018; no. 22 (6): 276–280 (in Russian). doi:10.18821/1728-2802-2018-22-6-276-280
13. Benson P.E., Parkin N., Dyer F., Millett D.T., Germain P. Fluorides for preventing early tooth decay (demineralised lesions) during fixed brace treatment. Cochrane Database Syst Rev. 2019; no. 2019 (11): CD003809. doi: 10.1002/14651858.CD003809.pub4
14. Fedorova K.V., Gavrilova O.A., Zatevalov A.M. Prediction of changes in the level of oral hygiene by API index when using different orthodontic appliances. Pediatric dentistry and dental profilaxis. 2024; no. 24 (2): 167–175 (in Russian). doi: 10.33925/1683-3031-2024-757
15. Taylor E.A, Donnelly E. Raman and Fourier transform infrared imaging for characterization of bone material properties. Bone. 2020; 139:115490. doi: 10.1016/j.bone.2020.115490
16.Fedorova K.V., Gavrilova O.A., Zatevalov A.M., Mironov A.Y., Samoylova M.V. Assessment of oral hygiene level by API index when using different orthodontic appliances. Certificate of registration of computer program RU 2023660603, 23.05.2023. Application № 2023619534 from 15.05.2023 (in Russian).
17. Samoylova M.V., Voropaeva E.A., Zatevalov A.M., Kosyreva T.F., Zhilenkova O.G., Tuturov N.S. et al. Prospectivity of the expansion of non-invasive methods of analysis for screening diagnostics (literature review). 2024; Epidemiology and infectious diseasesno. no. 29 (3): 170–176 (in Russian). doi: 10.51620/3034-1981-2024-29-3-170-176
18.Sedoykin A.G., Kiselnikova L.P., Zatevalov A.M., Ermoliev S.N., Fokina A.A. Application of autofluorescence microscopy and laser fluorometry to study the process of demineralization of temporary teeth in vitro. Stomatology. 2023; no. 102 (5): 6–13 (in Russian). doi: 10.17116/stomat20231020516
19.Aleksandrov M.T., Kukushkin V.I., Pashkov E.P., Kruglova L.V., Zuev V.M., Ambartsumyan O.A. et al. Device for Raman fluorescence diagnostics of human tissue condition in norm and pathology. Patent for useful model RU 144665 U1, 27.08.2014. Application No. 2013154656/14 of 10.12.2013 (in Russian).